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Small World Initiative: Research Protocols and Research Guide to Microbial and Chemical Diversity Package (two-book set)

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Small World Initiative: Research Protocols and Research Guide to Microbial and Chemical Diversity Package (two-book set)

4.85 85,720 ratings · 2,324 reviews
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Overview: This two-book set is part of the official materials used in the Small World Initiative® (SWI). Initiated at Yale University in 2012, SWI is an innovative program that inspires and retains students in the sciences by engaging them in the hunt to find new drugs to treat infectious diseases known as superbugs.'Infectious disease threats, like the coronavirus and superbugs, undermine modern medicine,' according to SWI's President, Erika Kurt. 'Leveraging the collective efforts of thousands of students and educators around the world gives us a real chance to tackle these challenges head on and provides an incredible STEM education in the process.' As COVID-19 has demonstrated, a lack of preparedness can take a huge human, financial, and educational toll, making the Small World Initiative's work critical to our fight against the next pandemic.Differentiating itself from traditional science education programs, SWI's program provides original research opportunities rather than relying on cookbook experiments with predetermined results. Students are introduced to discovery-based research through the core research project of antibiotic discovery from soil microbes. Through a series of experiments, students collect soil samples, isolate diverse bacteria, test their bacteria against clinically-relevant microorganisms, and characterize those showing inhibitory activity.While most antibiotics come from soil and it remains a great place to look for new ones, finding new candidates requires screening thousands of samples. SWI's approach provides the requisite platform to crowdsource antibiotic discovery by tapping into the power of thousands of student researchers concurrently addressing a global challenge. This unique approach harnesses the power of active learning to achieve both educational and scientific goals.Since its inception, SWI has impacted hundreds of schools across the United States and around the world and helps them transform science education and promote antibiotic discovery through the curiosity and inventiveness of young scientists. 'SWI allows students to not just dream about using science to save lives, but to actually dig in and help save the world, starting at school,' says Kurt. 'The program is an example of how groundbreaking research can start in the classroom.' If you are interested in learning more about the Small World Initiative, please visit www smallworldinitiative orgCONTENTSRESEARCH PROTOCOLSLab Safety and Best Practices // Agarose Gel Electrophoresis // Aligning and Combining 16S rRNA Gene Sequences // Analyzing Organic Extracts for Antibiotic Production // Analyzing Sequences with BLAST Search // Antibiotic Resistance Test // Catalase Test // Colony Morphology // Colony PCR // Fermentation // Gram Stain // MacConkey Agar Test // Making Glycerol Stocks // Methanol Extraction // Obtaining Soil Sample // Picking and Patching Colonies // Plating Soil Sample // Screen for Isolate Antibiotic Production #1 Patch/Patch // Screen for Isolate Antibiotic Production Patch #2 Spread/Patch // Screen for Isolate Antibiotic Production Patch #3 Top Agar // Serial Dilutions // Silica Column Chromatography Protocol // Spread Plate // Streak Plate // Sulfide and Indole Production and Motility // Thin Layer Chromatography (TLC) // Typical Media MenuRESEARCH GUIDESmall World Initiative® Overview // The Antibiotic Crisis // 1: Living on a Bacterial Planet // 2: More Than Just Dirt // 3: Redefining Growth and Culture // 4: Bacteria Are What They Eat, Too // 5: Solid Versus Liquid Cultures // 6: Meet the ESKAPE Pathogens // 7: Antibiotic Discovery, Structure, & Targets // 8: Getting to Know Your Isolates // 9: It All Comes Down to Chemistry // 10: Resisting Antibiotics // 11: Classic versus Modern // 12: Bacteria in Context // Future Directions

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Small World Initiative writes contemporary fiction and has always been fascinated by character-driven stories. They hold a degree in English Literature and previously worked as an editor before transitioning to writing their own books. Small World Initiative lives with their spouse and a very vocal rescue cat.

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Mark Martinez
Mark Martinez June 10, 2025
A highly sophisticated read. A brilliant concept executed with flair. Just a few pacing choices I would change. A very distinguished novel.
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Took a moment to build, but the payoff was spectacular. A brilliant concept executed with flair. Just a few pacing choices I would change. Quite impressive overall.
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Excellent, though slightly flawed. The protagonist was compelling, though some subplots felt slightly overshadowed. A wonderful companion for a quiet weekend.
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Honestly one of the most premium reading experiences lately. I adored how the story unfolded. It kept me captivated without sacrificing elegance. A true literary gem.
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A profoundly satisfying read. The author orchestrated a breathtaking finale. Truly a work of art. I am now a lifelong fan of this author.
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A highly sophisticated read. A brilliant concept executed with flair. Just a few pacing choices I would change. A wonderful companion for a quiet weekend.
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Took a moment to build, but the payoff was spectacular. Beautiful writing, though I craved slightly more depth in the lore. Still, undeniably engaging. A wonderful companion for a quiet weekend.
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Such a rich narrative. The prose is exquisite. It’s accessible yet undeniably sophisticated. I am now a lifelong fan of this author.
Matthew Wright
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Such a rich narrative. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. I am now a lifelong fan of this author.
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I thoroughly enjoyed the elegance of it. Beautiful writing, though I craved slightly more depth in the lore. Still, undeniably engaging. A wonderful companion for a quiet weekend.
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Absolutely brilliant. The prose is exquisite. It’s accessible yet undeniably sophisticated. An absolute must-read for connoisseurs of the genre.
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Exceeded all my expectations. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An effortless 5 stars.
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Honestly one of the most premium reading experiences lately. I became deeply invested in the protagonist's journey. A masterclass in storytelling. I am now a lifelong fan of this author.
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Absolutely brilliant. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. A true literary gem.
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I was completely blown away. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. Worth every moment spent reading.
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An acceptable diversion. It verged on repetitive in the second act. A more concise edit would have served it well. Suitable if your reading queue is empty.
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I thoroughly enjoyed the elegance of it. A brilliant concept executed with flair. Just a few pacing choices I would change. A solid 4 stars from me.
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I thoroughly enjoyed the elegance of it. The middle had a deliberate pace, but the climax was phenomenal. Quite impressive overall.
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Such a rich narrative. I adored how the story unfolded. It kept me captivated without sacrificing elegance. I will undoubtedly revisit this masterpiece.
George Green
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Such a rich narrative. The author orchestrated a breathtaking finale. Truly a work of art. A true literary gem.
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A profoundly satisfying read. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. I am now a lifelong fan of this author.
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Daniel Scott May 15, 2024
Honestly one of the most premium reading experiences lately. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. I am now a lifelong fan of this author.
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Exceeded all my expectations. The author orchestrated a breathtaking finale. Truly a work of art. Worth every moment spent reading.
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Such a rich narrative. The author orchestrated a breathtaking finale. Truly a work of art. I will undoubtedly revisit this masterpiece.
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A profoundly satisfying read. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. Worth every moment spent reading.
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A highly sophisticated read. A brilliant concept executed with flair. Just a few pacing choices I would change. A very distinguished novel.
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Such a rich narrative. The prose is exquisite. It’s accessible yet undeniably sophisticated. An effortless 5 stars.
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Took a moment to build, but the payoff was spectacular. The protagonist was compelling, though some subplots felt slightly overshadowed. Quite impressive overall.
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A profoundly satisfying read. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. An effortless 5 stars.
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Karen Harris October 08, 2025
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Exceeded all my expectations. I became deeply invested in the protagonist's journey. A masterclass in storytelling. An absolute must-read for connoisseurs of the genre.
Charles Ramirez
Charles Ramirez January 09, 2025
Honestly one of the most premium reading experiences lately. The pacing was impeccable and the characters felt incredibly authentic. I was completely immersed. I will undoubtedly revisit this masterpiece.
Karen Campbell
Karen Campbell April 14, 2025
I was completely blown away. I became deeply invested in the protagonist's journey. A masterclass in storytelling. I will undoubtedly revisit this masterpiece.
Ashley Brown
Ashley Brown May 29, 2024
A profoundly satisfying read. I became deeply invested in the protagonist's journey. A masterclass in storytelling. I am now a lifelong fan of this author.
Thomas Scott
Thomas Scott January 31, 2024
Such a rich narrative. The prose is exquisite. It’s accessible yet undeniably sophisticated. A true literary gem.
Rebecca Cruz
Rebecca Cruz May 12, 2024
Took a moment to build, but the payoff was spectacular. The protagonist was compelling, though some subplots felt slightly overshadowed. I would highly recommend this to discerning readers.
Jennifer Flores
Jennifer Flores April 01, 2024
A very strong entry. The middle had a deliberate pace, but the climax was phenomenal. I would highly recommend this to discerning readers.
Betty Garcia
Betty Garcia April 03, 2024
I thoroughly enjoyed the elegance of it. The middle had a deliberate pace, but the climax was phenomenal. A very distinguished novel.
William Moore
William Moore May 05, 2024
A profoundly satisfying read. I became deeply invested in the protagonist's journey. A masterclass in storytelling. An effortless 5 stars.
Betty Turner
Betty Turner March 28, 2025
A very strong entry. Beautiful writing, though I craved slightly more depth in the lore. Still, undeniably engaging. A wonderful companion for a quiet weekend.
Thomas Scott
Thomas Scott November 03, 2024
A highly sophisticated read. The protagonist was compelling, though some subplots felt slightly overshadowed. A very distinguished novel.
Sarah Thomas
Sarah Thomas February 02, 2024
A highly sophisticated read. Beautiful writing, though I craved slightly more depth in the lore. Still, undeniably engaging. A wonderful companion for a quiet weekend.
Mary Hill
Mary Hill November 06, 2025
Exceeded all my expectations. I became deeply invested in the protagonist's journey. A masterclass in storytelling. Worth every moment spent reading.
Paul King
Paul King November 23, 2024
Honestly one of the most premium reading experiences lately. The prose is exquisite. It’s accessible yet undeniably sophisticated. Worth every moment spent reading.
Nicholas Harris
Nicholas Harris July 30, 2024
A profoundly satisfying read. The author orchestrated a breathtaking finale. Truly a work of art. I am now a lifelong fan of this author.
Charles Lopez
Charles Lopez June 17, 2025
Masterpiece from start to finish. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. I am now a lifelong fan of this author.
Charles Garcia
Charles Garcia September 18, 2024
Excellent, though slightly flawed. The middle had a deliberate pace, but the climax was phenomenal. Quite impressive overall.
Nancy Miller
Nancy Miller November 20, 2025
I was completely blown away. The prose is exquisite. It’s accessible yet undeniably sophisticated. I am now a lifelong fan of this author.
Kimberly Hall
Kimberly Hall August 27, 2025
A profoundly satisfying read. I adored how the story unfolded. It kept me captivated without sacrificing elegance. I am now a lifelong fan of this author.
David Hill
David Hill March 17, 2024
Exceeded all my expectations. The author orchestrated a breathtaking finale. Truly a work of art. An absolute must-read for connoisseurs of the genre.
Susan Cruz
Susan Cruz July 16, 2024
Masterpiece from start to finish. I adored how the story unfolded. It kept me captivated without sacrificing elegance. Worth every moment spent reading.
Joseph Johnson
Joseph Johnson September 12, 2024
Excellent, though slightly flawed. The middle had a deliberate pace, but the climax was phenomenal. A solid 4 stars from me.
George Gomez
George Gomez October 04, 2024
An acceptable diversion. It possessed moments of brilliance, but the narrative felt slightly disjointed ultimately. A fair 3 stars.
Paul Rodriguez
Paul Rodriguez May 09, 2025
A highly sophisticated read. A brilliant concept executed with flair. Just a few pacing choices I would change. A wonderful companion for a quiet weekend.
Brian Hall
Brian Hall September 14, 2025
Exceeded all my expectations. The author orchestrated a breathtaking finale. Truly a work of art. An absolute must-read for connoisseurs of the genre.
Joseph Lopez
Joseph Lopez October 26, 2025
Masterpiece from start to finish. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An effortless 5 stars.
Lisa Mitchell
Lisa Mitchell June 07, 2025
A profoundly satisfying read. I became deeply invested in the protagonist's journey. A masterclass in storytelling. An effortless 5 stars.
Andrew Perez
Andrew Perez July 02, 2024
An acceptable diversion. It verged on repetitive in the second act. A more concise edit would have served it well. Unlikely to reread, but a reasonable expenditure of time.
John Jones
John Jones February 05, 2024
Such a rich narrative. I adored how the story unfolded. It kept me captivated without sacrificing elegance. I am now a lifelong fan of this author.
Margaret Walker
Margaret Walker March 29, 2024
Absolutely brilliant. It strikes a perfect harmony of dialogue and world-building. Every chapter felt necessary. An effortless 5 stars.
Nicholas Hill
Nicholas Hill August 19, 2024
Not entirely to my refined tastes. The prose was competent, yet the characters lacked the depth I usually seek. Suitable if your reading queue is empty.
Kenneth Martinez
Kenneth Martinez November 09, 2025
I was completely blown away. The prose is exquisite. It’s accessible yet undeniably sophisticated. Worth every moment spent reading.
David Allen
David Allen April 22, 2025
I was completely blown away. The author orchestrated a breathtaking finale. Truly a work of art. A true literary gem.
Donna Johnson
Donna Johnson July 17, 2025
Absolutely brilliant. The author orchestrated a breathtaking finale. Truly a work of art. Worth every moment spent reading.
Mark Cruz
Mark Cruz July 04, 2024
Excellent, though slightly flawed. Beautiful writing, though I craved slightly more depth in the lore. Still, undeniably engaging. A wonderful companion for a quiet weekend.
Michael Turner
Michael Turner September 17, 2024
Excellent, though slightly flawed. It held my rapt attention, even when certain twists were telegraphed. A wonderful companion for a quiet weekend.
William King
William King November 21, 2025
Absolutely brilliant. I adored how the story unfolded. It kept me captivated without sacrificing elegance. An absolute must-read for connoisseurs of the genre.
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